Evaluation of anthocyanin extract of Thai Morus ALBA L., on blood glucose level in diabetic rats and prediabetes patients

Name: Ariya Sarikaphuti

Organization : Mae Fah Luang University

keyword: Thai Morus ALBA L.

LCSH: Plants, Medicinal

Classification :.LCCS: QV766

LCSH: Plant Extracts

LCSH: Thai Morus ALBA L.

Abstract: Mulberry (Molus alba L.) contains abundant anthocyanin (ANC), a natural antioxidant. The purpose of this study was to determine the ANC composition of Thai Morus alba L. fruits, and to assess its effect on blood glucose and insulin levels in ZDF rats and pre diabetes patients. In this study the major components of ANC were identified by high-performance liquid chromatography–electrospray ionization–mass spectrometry. ZDF and lean rats were administered with 125 or 250 mg ANC/kg body weight, or 1% carboxymethylcellulose (CMC) twice daily for 5 weeks. Neither ANC dose affected body weight. After 5 weeks of treatment, glucose levels increased from 105.5 ± 8.7 to 396.25 ± 21 mg/dl (P < 0.0001) in CMC-treated ZDF rats, but levels were significantly lower in rats treated with 125 (228.25 ± 45 mg/dl) or 250 (131.75 ± 10) mg/kg ANC (P < 0.001 vs. CMC). Administration of 250 mg/kg ANC normalized glucose levels in ZDF rats towards those of lean littermates. Insulin levels decreased significantly in ZDF rats treated with CMC or 125 mg/kg ANC (P < 0.0001), but not in rats treated with 250 mg/kg ANC. Histologically, 250 mg/kg ANC prevented islet degeneration compared with islets in CMC-treated rats. We confirmed that ANC was well tolerated and had effective antidiabetic properties in male leptin receptor-deficient Zucker diabetic fatty (ZDF) rats. In human study, the effectiveness of freeze dried mulberry fruit of Thai Morus alba L., on fasting blood sugar and HbA1C in pre diabetes patients were evaluated. Participants included 18 pre diabetes patients. They were divided into 2 groups (n=9). Subjects ingested either a capsule containing ANC or gelatin 750 mg. for 2 times a day. Fasting blood sugar (FBS), haemoglobin A1C (HbA1C), body weight, and body mass index (BMI) were assessed before and at intervals of 4 weeks for 12 weeks. After 12 weeks of treatment, FBS decreased significantly in pre diabetes patients treated with ANCs from 112.8 + 5.5 mg/dl to 93.0 + 10.45 mg/dl (p=0.0001), but not in the patients treated with gelatin (p= 0.1). Moreover, HbA1c also decreased in pre diabetes patients treated with freeze dried mulberry from 5.87 + 0.13 % to 5.46 + 0.37 (p=0.006) but not in the patients treated with gelatin (p=0.43). Here, we show for the first time that ANCs extracted from Thai Morus alba L. are an important antidiabetic agent. Furthermore, the ANC extract appeared to prevent the pathogenic lesions in diabetic islets by suppressing islet degeneration. We conclude that ANC may represent a promising class of therapeutic compounds that can be useful in type 2 diabetes prevention.

Mae Fah Luang University. The Learning Resources and Education Media Center

Address: Chiang Rai

Email: library@mfu.ac.th

Name: Thamthiwat Nararatwanchai

Role: Advisor

Name: Salunya Tancharoen

Role: Co-Advisor

Created: 2012

Issued: 2560

Modified: 2020-05-01

Issued: 2017-10-04

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Thesis QV766 A718e 2012

eng

DegreeName: Dotor of Philosophy

Level: Doctoral Degree

Descipline: Anti-Aging and Regenerative Science

Grantor: Mae Fah Luang University

©copyrights Mae Fah Luang University

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