Effect of plant growth regulators on gene expression and flower development in jatropha curcas l.

Name: Anupharb Seesangboon

Organization : Mae Fah Luang University

keyword: Plant growth regulators

LCSH: Plant genetics

Classification :.LCCS: QK981

LCSH: Plant regulators

LCSH: Plant biotechnology

LCSH: Plant Growth Regulators

Abstract: Jatropha curcas L. has been considered as a potential source of biodiesel feedstock due to their highly seed oil content (~40%). However, Jatropha exhibits a low number of female flowers, lead to insufficient support of their seed yield for biodiesel production. This problem can be addressed by the application of 6- benzyladenine (BA) and Paclobutrazol (PCB) on the Jatropha. However, the underline molecular mechanism of how BA and PCB effect remain unclear. Therefore, in this study aimed to investigate the effects of BA and PCB on flower development and gene expression of the Jatropha. It was found that, the application of BA and PCB can be used to increasing female number and seed yield of the Jatropha. Triple treatment of BA was significantly induced female flower and seed yield through increasing of number and distance of the pedicel (p<0.05). Moreover, only single PCB treatment was increased the Jatropha female flower and seed yield trough decreasing of gibberellin (GA) activity. The morphological study of flower development using SEM revealed that Jatropha flower buds spending 24 h from the first treatment for changing its stage of development. Moreover, there were no different of developmental stages between those three treatments. However, BA and PCB might be regulated the floral development mechanism in the molecular level. Therefore, flower buds at 0h, 4h, 24h were selected based on the presence of sex organs for investigating of genes involved in flower development and identifying sex determination gene in Jatropha using qRTPCR and RNA-Seq. Gene expression analysis and statistical analysis shows that, BA stimulated various of that might be involved in flower development. Interestingly, through BA application, gene that might be involved in female flower formation including SUPERMAN (JcSUP), TASSELSEED2 (JcTS2), and CYTOKININ OXIDASE/DEHYDROGENASE (JcCKX5). Therefore, those genes might play an important role to enhance the Jatropha female floral formation by increasing the arrest of stamens and enhance the inflorescences area through cytokinin activity. Moreover, gene expression analysis after PCB treatment revealed that, PCB inhibit GA activity passed through GA biosynthesis genes. Surprisingly, increasing number of female florals by the PCB-treatment might be mediated the expression of JcTS2, and JcCKX5. Moreover, transcriptomic analysis after PCB treatment shown that, there were several of hormones that mainly regulated floral development, including GA, auxin, ethylene, jasmonate, and abscisic acid. This study presents the report of morphological study of Jatropha flower bud developmental stage according to the time of chemical treatment and global gene expression pattern of Jatropha flower buds after PCB treatment by RNA-Seq. Therefore, this study could be used as genetics information for further mechanisms studies in Jatropha or other plant model for high yielding transgenic plant.

Mae Fah Luang University. The Learning Resources and Education Media Center

Address: Chiang Rai

Email: library@mfu.ac.th

Name: Siam Popluechai

Role: Advisor

Name: Jantrararuk Tovaranonte

Role: Co-Advisor

Created: 2016

Issued: 2560

Modified: 2020-04-29

Issued: 2017-08-16

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Thesis QK981 A636e 2016

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biotechnology

Grantor: Mae Fah Luang University

©copyrights Mae Fah Luang University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	Anupharb Seesangboon.pdf	30.03 MB	35	2024-03-26 11:56:56

ใช้เวลา

0.040309 วินาที