Control of mango anthracnose by using Chinese quince (pseudocydonai sinensis) seed extract

Name: Angraeni, Lia

Organization : Mae Fah Luang University

keyword: Mango anthracnose

LCSH: Plant diseases

Classification :.LCCS: SB732.5

Abstract: Anthracnose disease (caused by Colletotrichum gloeosporioides (Penz.) Sacc.) is the most important postharvest disease in mango. The alternative natural compound is explored to reduce the use of chemical pesticide for controlling the disease. Chinese quince (Pseudocydonia sinensis Schneid.) has been known in its rich in phenolic compounds and has been reported in its anti-inflammatory effects. However, Chinese quince seed is usually a waste which may also contain high phenolic compound. The objectives of this study were to investigate the potential in vitro and in vivo antimicrobial activity of Chinese quince seed extract against anthracnose disease in mango and to identify the phenolic profile of the extract. The seeds were extracted by using distilled water in ratio 1:10 (seed : water, w/v) and tested at different concentrations. In vitro antimicrobial activities were observed through mycelial radial growth and spore germination assays. Colletotrichum sp. was grown on the agar amended with Chinese quince seed extract at concentrations of 0.5%, 1% and 1.5%. Extract at all concentrations significantly inhibited (p < 0.05) mycelial growth of Colletotrichum sp. compared to the control. The 1.5% extract showed mycelial growth inhibition at 82.59% after 5 days of incubation, slightly lower than the fungicide Carbendazim did (100%), while 0.5% and 1% extract had lower inhibitory effect (78.85% and 78.41%, respectively). There was no mycelial growth inhibition observed in untreated control. Spore germination assay was carried out by using cavity slide technique. Ten µL of Colletotrichum sp. spore suspension at concentration of 105 spores/mL was placed on the slide, and then 40 µL of extract was added. Spore germination in untreated control (23.68%) was significantly less (p < 0.05) than that in 0.5%, 1% and 1.5% extracts (61.31%, 52.86% and 55.75%, respectively). This study indicated that Chinese quince seed extract was effective to inhibit Colletotrichum sp. mycelial growth under in vitro but did not inhibit fungal spore germination. The efficacy of Chinese quince seed extract for controlling anthracnose disease was tested on mango fruits (in vivo). The mango fruits were artificially inoculated with Colletotrichum sp. and were then dipped into different concentrations of extract solution. Mango fruit treated with 1% extract exhibited smaller lesion diameter (1.36 cm) compared to those treated with 0.5% and 1.5% extract (1.52 cm and 1.43 cm, respectively) and control (2.17 cm) after 5 days of incubation. The lesion diameter of fruits in all treatments increased during the storage period. At the end of storage period, control fruit exhibited lesion diameter at 2.88 cm, significantly higher (p < 0.05) than fruits treated by extracts and 0.1% Carbendazim (0.96 cm). It is suggested that Chinese quince seed extract has potential to control anthracnose disease on mango. Chinese quince seed extract was used to made edible film at different concentrations. The films were tested for its antifungal activity against Colletotrichum sp. by placing the film disc on the agar inoculated with 40 µL of Colletotrichum sp. spore suspension at concentration of 105 spores/mL. There was no clear inhibitory zone formed surrounding the film disc after 7 days of inoculation, suggesting that Chinese quince seed film did not inhibit the growth of Colletotrichum sp. The total phenolic content of the extract was measured and phenolic profile of the extract was identified by using HPLC and LC-ESI-MS system. The total phenolic content in Chinese quince seed extract measured with the Folin-Ciocalteu method was 48.84 ± 1.71 mg GAE/ 100 g wet sample. LC-ESI-MS analysis showed that Chinese quince extract contained lucenin-2, vicenin-2, isoschaftoside and schaftoside as its phenolic compound.

Mae Fah Luang University

Address: CHIANG RAI

Email: library@mfu.ac.th

Name: Matchima Naradisorn

Role: Advisor

Name: Wirongrong Tongdeesoontorn

Role: Co-Advisor

Modified: 2020-04-27

Created: 2016

Issued: 2017-03-13

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Thesis SB732.5 A593c 2016

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Technology Management of Agricultural Produces

Grantor: Mae Fah Luang University

©copyrights Mae Fah Luang University

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