Abstract:
Diets play important roles in determining maturation of broodstock. The major natural diets of shrimp broodstock are bivalves, squids and polycheates. Current research found that polycheate has been used successfully as a feed source to produce maturation in broodstock. It has been suggested that certain highly unsaturated fatty acids in polycheate may help trigger maturation. The purpose of this study was to develop a formula diet using similar fatty acid composition as the polycheate and evaluate the diet on female maturation ; ovary and eggs development, the values of gonad-somatic index (GSI), heptopancreas index (HI). The experiment was tested using 4 dietary treatments : (1) Commercial broodstock diets. (2) Nature food containing ; squid, oyster and polycheates. (3) The practical diets formulated in this study. (4) The alteration of practical diet natural food in a ratio of 50150 .The experimental periods is 22 days at salinity 30 ppt. The results showed that, the bloodworm had a ratio of fatty acid of AA : EPA : DHA as 5.8 : 5.6 : 1, respectively. For the ratios of the diet formula that constructed in this study and the commercial diet formula were 5 : 1 : 1 and 1 : 6.5 : 5.4, respectively . After 22 days of the experiment, only female shrimps fed nature food and alternation of practical diet and natural food (50/50) reached stage IV of development. Its hads the percent of ovary in cortical rod and the fatty acid analysis of AA, EPA and DHA in hepatopacrease and muscle were no significant. AA and EPA in ovary was no significant, except; DHA in ovary was significant.