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Angkana Chaipuang. Synthesis of molecular imprinted polymers for extraction and determination of flavonoids in tea leaves. Master's Degree(Applied Chemistry). Mae Fah Luang University. The Learning Resources and Education Media Center. : Mae Fah Luang University , 2015.
Synthesis of molecular imprinted polymers for extraction and determination of flavonoids in tea leaves
Name: Angkana Chaipuang
Organization :
Mae Fah Luang University
keyword:
Tea leaves
LCSH:
Polymer chemistry
Classification :.LCCS: QD382.I43
LCSH:
Polymers
LCSH:
Chemistry
LCSH:
Materials science
LCSH:
Molecular imprinting
Abstract:
Molecular imprinted polymers synthesized using quercetin and (+)-catechin flavonoids as the imprinting molecules were applied for the extraction of the flavonoids from the fermented tea leaves. The polymers have been successfully synthesized by bulk and solution polymerization techniques using methacrylic acid and acrylamide as functional monomer and ethylene glycol dimethacrylate and pentaerythriol triacrylate as crosslinker. The solution of acetonitrile was used as porogen. Systematic evaluations of the influence of monomer, crosslinker, porogen, and polymerization conditions over the properties of the MIPs have been carried out. The quercetin MIPs and (+)-catechin MIPs were evaluated for their adsorption and kinetic characteristics as well as their selective recognition properties for their imprinting compounds and structurally related compound (kaempferol). Chemical and morphological characterization of the MIPs was investigated by FTIR, SEM, SEM-EDX, TEM, BET, and TGA, which comfirmed the high degree of polymerization. Accordingly, the synthesized MIPs were used as a solid-phase extraction sorbent for the extraction and enrichment of quercetin and (+)-catechin in the fermented tea leave samples, followed by fluorescence spectrometry and HPLC-DAD analysis. Quercetin concentrations of 5.63 77.64 ppm and 56.40 953.07 ppm were determined by HPLC and fluorescence spectrometry, respectively. (+)-Catechin concentrations of 229.75 - 17349.06 ppm and 104.68 5516.00 ppm were determined by HPLC and fluorescence spectrometry, respectively. The method validation of the proposed fluorescence spectrometry technique was compared to HPLC technique. From the results of the comparison between the two analysis techniques, T-test value is not significant difference at 98 % confidence level. However, F-test value showed that (+)-catechin analysis is not significant difference at 99 % confidence level, but F-test value of determination of quercetin is significant difference at 99 % confidence level. The linearity of working range of (+)-catechin and quercetin which were investigated by fluorescence spectrometry method are in the range of 3.0-20.0 ppm with LOD and LOQ defined equal to 2.28, 2.04, 2.43 and 2.29 ppm, respectively. The mean recoveries were 90.08, 112.27, 83.65 and 126.22 % (n = 3) of (+)-catechin and quercetin determined by HPLC and fluorescence spectrometry method, respectively.
Mae Fah Luang University. The Learning Resources and Education Media Center
Address:
Chiang Rai
Email:
library@mfu.ac.th
Name: Theeraphan Machan
Role:
Advisor
Created:
2015
Issued:
2016
Modified:
2020-04-27
Issued:
2016-12-22
วิทยานิพนธ์/Thesis
application/pdf
CallNumber:
Thesis QD382.I43 A588s 2015
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Applied Chemistry
Grantor: Mae Fah Luang University
©copyrights Mae Fah Luang University
RightsAccess:
ใช้เวลา
0.019669 วินาที
Angkana Chaipuang
| Title | Contributor | Type |
|---|---|---|
| Synthesis of molecular imprinted polymers for extraction and determination of flavonoids in tea leaves
มหาวิทยาลัยแม่ฟ้าหลวง Angkana Chaipuang | Theeraphan Machan, Ph. D. | วิทยานิพนธ์/Thesis |
| Comparative study on two polymerization methods for synthesis of copper(II) ion imprinted polymers
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Angkana Chaipuang;Chalida Phungpanya;Chalermporn Thongpoon;Kanchana Watla-iad;Orawan Suwantong;Prachak Inkaew;Theeraphan Machan | บทความ/Article | |
| Preparation of magnetic molecularly imprinted polymer for glutathione
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Monnapas Wongtipakorn;Angkana Chaipuang;Chalida Phungpanya;Chalermporn Thongpoon;Theeraphan Machan;Kanchana Watla-iad | บทความ/Article | |
| Synthesis and application of copper ion imprinted polymers
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