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Nouanthavong, Souksanh. Development of a paper-based microfluidic device for pesticide analysis in dried seafood using nanoceria as colorimetric probes. Master's Degree(Chemical Education). Burapha University. Library. : Burapha University, 2015.
Development of a paper-based microfluidic device for pesticide analysis in dried seafood using nanoceria as colorimetric probes
Name: Nouanthavong, Souksanh
keyword:
Nanoceria
Abstract:
We report the first use of paper-based device coated with nanoceria as a
detection platform for the analysis of organophosphate (OP) pesticides using enzyme
inhibition assay to allow for simple, low-cost and rapid analysis OPs in the samples.
The detection assay consisted of nanoceria, acetylcholinesterase (AChE), and choline
oxidase (ChOX). In the presence of acetylcholine, the enzyme AChE and ChOX
catalyze the formation of H2O2, which is then detected colorimetically by nanoceriacoated
paper-based device producing yellow color. After incubation with OP
pesticides, the activity of AChE was inhibited and produced less H2O2, hence, causing
a drop of yellow color intensity. Color intensity was analyzed on the scanned picture
using ImageJ software. This developed assay is able to analyze OP pesticides without
the use of any external sophisticated instruments with excellent detection limits of
two model OP pesticides where as low as 18.3 ng/mL of methyl-paraoxon and 5.2 ng/mL
of chlorpyrifos-oxon were observed. The developed method has been successfully
applied to detect methyl-paraoxon in spiked vegetables (cabbage) and dried seafood
product (dried green mussel), obtaining recovery values about 95% for both types of
samples. The spiked samples were also analyzed using LC-MS/MS as a comparison
to the developed method and similar values were obtained indicating that the
developed method is validated and suitable for OP analysis in real samples. Therefore,
the developed assay is promising method to use as a new alternative method for
analysis of OP pesticides that provides low-cost, rapid, and onsite OPs analysis.
Burapha University. Library
Address:
CHONBURI
Email:
buulibrary@buu.ac.th
Name: Yupaporn Sameenoi
Role:
Principal advisor
Created:
2015
Modified:
2565-07-25
Issued:
2016-01-06
วิทยานิพนธ์/Thesis
application/pdf
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Chemical Education
Grantor: Burapha University
ใช้เวลา
0.020392 วินาที
Nouanthavong, Souksanh
| Title | Contributor | Type |
|---|---|---|
| Development of a paper-based microfluidic device for pesticide analysis in dried seafood using nanoceria as colorimetric probes
มหาวิทยาลัยบูรพา Nouanthavong, Souksanh | Yupaporn Sameenoi | วิทยานิพนธ์/Thesis |
Yupaporn Sameenoi