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Sawinee Seemakhan. In vitro anti-inflammatory activity of some medicinal plants from ban ang-ed official community forest, chanthaburi province. Master's Degree(Biological Science). Burapha University. Library. : Burapha University, 2015.
In vitro anti-inflammatory activity of some medicinal plants from ban ang-ed official community forest, chanthaburi province
Name: Sawinee Seemakhan
keyword:
Inflammation
Abstract:
Ban Ang-Ed community forest, Chanthaburi Province (the Chaipattana
Foundation) is biodiversed with flora and fauna. The present study aims to evaluate
anti-inflammatory activity of selected medicinal plants from Ban Ang-Ed official
community forest. Twenty-one medicinal plants were extracted with water and
ethanol. The anti-inflammatory activity was determined by measuring an inhibitory
effect on inducible nitric oxide synthase (iNOS)-catalyzed nitric oxide (NO) and
cyclooxygenase-2 (COX-2)-catalyzed prostaglandin E2 (PGE2) production in
lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Cell viability of
macrophages exposed to the plant extracts was determined by MTT assay. The results
showed that all selected medicinal plants exhibited anti-inflammatory activity via
inhibition of NO and PGE2 production. Among them, the ethanol extract from
Ageratum conyzoides Linn leaves and Clausena excavate Burm F. roots showed high
anti-inflammatory activity with no significant cytotoxicity. The ethanol extract of
A.conyzoides leaves (ACE) exhibited an inhibitory effect on production of NO and
PGE2 with IC50 values of 22.47±0.22 µg/mL and 26.89±1.73 µg/mL, respectively.
ACE attenuated the expression of iNOS and COX-2 at mRNA and protein levels in a
concentration-dependent manner. Moreover, ACE suppressed the level of nuclear
factor-κB (NF-κB) p65 translocation and phosphorylation of c-Jun NH2-terminal
kinases (JNK) and extracellular receptor kinase (ERK) of mitogen-activated protein
kinases (MAPKs). Further, ACE was sequentially extracted by hexane, ethyl acetate
and water fraction. The ethyl acetate fraction of ACE was further isolated by silica gel column chromatography. Among the 10 resultant sub-fractions, F8-F10 exhibited
significant anti-inflammatory activity without cytotoxicity. These results indicate that
ACE inhibits inflammation by suppress NO and PGE2 production through
suppression of iNOS and COX-2 expression via a signaling pathway that involves
NF-κB nuclear translocation and JNK and ERK phosphorylation. These findings
provide the scientific evidence to justify the anti-inflammatory therapeutic use of the
selected medicinal plants from Ban Ang-Ed official community forest including
A.conyzoides in traditional medicine.
Burapha University. Library
Address:
CHONBURI
Email:
buulibrary@buu.ac.th
Name: Klaokwan Srisook
Role:
Principal advisor
Name: Ekaruth Srisook
Role:
Co-advisor
Created:
2015
Modified:
2564-11-16
Issued:
2015-10-22
วิทยานิพนธ์/Thesis
application/pdf
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Biological Science
Grantor: Burapha University
ใช้เวลา
0.024483 วินาที
Sawinee Seemakhan
| Title | Contributor | Type |
|---|---|---|
| In vitro anti-inflammatory activity of some medicinal plants from ban ang-ed official community forest, chanthaburi province
มหาวิทยาลัยบูรพา Sawinee Seemakhan | Klaokwan Srisook Ekaruth Srisook | วิทยานิพนธ์/Thesis |
Klaokwan Srisook
Ekaruth Srisook