Abstract:
ABSTRACT
Experiment 1 The aim of this research was study the properties and the quality of fermented herbs fruits and mixed juice to used Lactobacillus plantarum and Lactobacillus brevis in powder bacterium form, that produces lactic acid . The fermented products were prepared from 8 local herbs, namly, Turmeric ( Curcuma longa), Betel (Piper betle), Kariyat (Andrographis paniculata),Siam weed (Eupotorium odortum), Aloe (Aloe vera), Climacanthus nutans, Turnsole (Heliotropium indicom ) and Asiatic Pennywort (Centella asiatica) by controlled fermentation process. The fermented juice composed of 3 part of herb, 10 part of water, 1 part of sugar and 250 ml of lactic acid bacteria. All ingredients were poured into fermenter tank immediately immersed in an ice bucket (cool shock) by air lock (air Lock) system. Results showed a good quality. The analysis found that the concentration of alcohol content was between 0.4-1.1%. The acidity (pH) was different according to kinds of herb: Turmeric fermented juice had optimum pH 3.2-3.9 at 30 day of fermentation. Betel and Kariyat provided pH 3.2 . The good quality fermented juice had brown and cloudy appearance. The smell and taste were sour. All kinds of fermented juice showed safety standards.
Experiment II The study was to determine inhibition efficiency of the fermented juices of local medicinal thai plants against Staphylococcus aureus.This research aims to investigate the inhibition efficiency of 8 fermented juices of local medicinal Thai plants against Staphylococcus aureus, which normally causes cow mastitis. The local plants used in this study were Turmeric ( Curcuma longa), Betel (Piper betle), Kariyat (Andrographis paniculata),Siam weed (Eupotorium odortum), Aloe (Aloe vera), Climacanthus nutans, Turnsole (Heliotropium indicom ) and Asiatic Pennywort (Centella asiatica). The paper-disc diffusion method was used to compare inhibition efficiency between the fermented juices of local medicinal Thai plants and antibiotic (oxytetracyclin). The result showed that 2-month old of the fermented juice of Eupotorium odortum L. with Lactobacillus plantarum displayed the highest inhibition efficiency against S. aureus. The diameter of clear zone was 0.867±0.166 cm. and its inhibition efficiency was comparable to the antibiotic efficiency when using 50 g of antibiotic/disc as about 66.67%.
Experiment III The effect of fermented herbs juice on somatic cell count and immunity in periparturient dairy cows . This experiment was conducted to study using of fermented herds juice, fermented fruits juice and mixed juice. The experiment was conducted according to a Randomized Complete Block Design, RCBD in 75% hybrid Holstein Friesian in periparturient dairy cows, twenty dried off dairy cows were assigned into 2 groups: dairy cows with no fermented juice, and dairy cows with fermented herb juice, fermented fruit juice and mixed juice mixed concentrate fed during 13 days before parturition to 14 days after parturition. Experimental results showed that dairy cows from control groups had 80% postpartum mastitis and highly somatic cell count. The dairy cows on fermented herb juice groups had lower somatic cell count than other groups and high milk fat and protein at 4.5% and 3.28%, respectively. Dairy cows had similar hematologic profile except WBC at 14 days after parturition. The dairy cows on fermented fruits juice groups had high somatic cell count and caused to 40% % postpartum mastitis. Dairy cows received fermented juice had higher milk yield than other groups and no mastitis.
Keyword: Fermented juice Herb Fruit Dairy cow Mastitis