ÃÒ§ҹ¡ÒÃÇÔ¨ÑÂÃкºµÃǨÇÑ´ÃдѺ¤ÇÒÁäǵèÍÂÒµéÒ¹¨ØŪվÍѵâ¹ÁѵÔ

Automated antimicrobial susceptibility testing system

ÃкºµÃǨÇÑ´ÃдѺ¤ÇÒÁäǵèÍÂÒµéÒ¹¨ØŪվÍѵâ¹ÁѵÔ

Name: ÊØþŠÈÃպح·Ã§

Organization : ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕ¾ÃШÍÁà¡ÅéÒ¾Ãй¤Ãà˹×Í.

Email : sss@kmutnb.ac.th

keyword: Disc diffusion technique

ThaSH: ¡Ò÷´Êͺ¤ÇÒÁäǢͧ¨ØŪվ

Classification :.DDC: QR69.A57

; §Ò¹ÇԨѤ³ÐÇÔ·ÂÒÈÒʵÃì»ÃÐÂØ¡µì

ThaSH: ÂÒ -- ¼Å¡Ãзº·Ò§ÊÃÕÃÇÔ·ÂÒ

ThaSH: àǪÀѳ±ì -- ¡ÒÃÍ͡ẺáÅСÒÃÊÃéÒ§

Noted: §Ò¹ÇԨѹÕéä´éÃѺ·Ø¹Ê¹ÑºÊ¹Ø¹¨Ò¡·Ø¹§º»ÃÐÁÒ³á¼è¹´Ô¹ ¨Ò¡ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕ¾ÃШÍÁà¡ÅéÒ¾Ãй¤Ãà˹×Í »ÃÐ¨Ó»Õ 2550.

Abstract: âäµÔ´àª×éͨØŪվà»ç¹»Ñ­ËÒÊØ¢ÀÒ¾ÊӤѭÍÂèҧ˹Ö觢ͧ»ÃÐà·Èä·Â«Öè§ÍÒ¨ä´éÃѺ ¡ÒäǺ¤ØÁÍÂèÒ§ä´é¼Å´éÇÂÂÒ»¯ÔªÕǹÐâ´ÂÍÒÈÑ¡Ò÷´Êͺ¤ÇÒÁäǵèÍÂÒã¹ÃٻẺµèÒ§æ ¡Ñ¹ ˹Öè§ã¹ÇÔ¸Õ·´Êͺ·Õèä´éÃѺ¤ÇÒÁ¹ÔÂÁã¹Ëéͧ»¯ÔºÑµÔ¡ÒèØŪÕÇÇÔ·ÂÒ¤×Íà·¤¹Ô¤ Kirby Bauer’s Disc Diffusion Test à¹×èͧ¨Ò¡à»ç¹à·¤¹Ô¤·ÕèÁյ鹷عµèÓ ÊÒÁÒö·´Êͺ¤ÇÒÁäǵèÍÂÒ»¯ÕǹÐä´é·ÕÅÐ ËÅÒª¹Ô´ áÅÐÁÕá¹Çâ¹éÁ㹡ÒþѲ¹Òä»ÊÙè¡Ãкǹ¡Ò÷´ÊͺÍѵâ¹ÁѵԷÕèÁÕ¤ÇÒÁ¶Ù¡µéͧáÅÐ áÁè¹ÂÓ¡ÇèÒ¡Ò÷´Êͺ´éÇÂÊÒµÒÁ¹ØÉÂìä´é ÊÒÁÒöŴ»Ñ­ËÒ¤ÇÒÁ¼Ô´¾ÅÒ´Íѹà¹×èͧÁÒ¨Ò¡ ¤ÇÒÁᵡµèҧ㹡ÒÃÍèÒ¹áÅÐá»Å¼Å¢Í§¼Ùé·´ÊͺáµèÅÐÃÒ Ŵ¤ÇÒÁᵡµèҧ㹡Ò÷´Êͺáµè ÅФÃÑé§ Å´¤ÇÒÁ«éÓ«é͹áÅÐàÁ×èÍÂÅéҢͧ¼ÙéàªÕèÂǪҭàÁ×èÍÁըӹǹÊÔè§Ê觵ÃǨÁÒ¡ áÅÐÊÒÁÒöÊè§ µèÍ¢éÍÁÙżšÒ÷´Êͺà¢éÒÊÙèÃкººÃÔËÒèѴ¡ÒÃâç¾ÂÒºÒÅã¹ÃÙ»¢éÍÁÙÅÍÔàÅç¡·Ã͹ԡÊìä´éâ´ÂµÃ§ ÃкºµÃǨÇÑ´ÃдѺ¤ÇÒÁäǵèÍÂÒµéÒ¹¨ØŪվ·Õèä´éÃѺ¡ÒÃÍ͡Ẻ¹Õé »ÃСͺ仴éÇ ÍØ»¡Ã³ìÍèÒ¹ÀÒ¾¨Ò¹àÅÕé§àª×éÍà¾×èÍÍèÒ¹áÅÐá»Å¼Åà»ç¹¤ÇÒÁäÇ ËÃ×Í ´×é͵èÍÂÒ»¯ÔªÕǹШҡ¢¹Ò´ àÊé¹¼èÒ¹ÈÙ¹Âì¡ÅÒ§¢Í§¢Íºà¢µÂÑ駡ÒÃà¨ÃÔ­àµÔºâµ¢Í§àª×éͨØŪվ â´ÂÍÒÈÑ¡Ãкǹ¡ÒûÃÐÁǼŠÊÑ­­Ò¹ÀÒ¾ÍÒ·Ôàªè¹¡ÒûÃѺÀÒ¾ãËéàÃÕº ¡ÒÃà¹é¹¢ÍºÀÒ¾ ¡Òäé¹ËÒ¾×é¹·ÕèáÅТͺÀÒ¾·Õèà¡Ô´ ¨Ò¡ÒÃÂѺÂÑ駡ÒÃà¨ÃÔ­àµÔºâµ¢Í§¨ØŪվ ÊÒÁÒöàª×èÍÁµèÍà¢éҡѺÃкººÃÔËÒâéÍÁÙÅËéͧ»¯ÔºÑµÔ¡Òà ¨ØŪÕÇÇÔ·ÂÒ MLAB system «Öè§ä´éÃѺ¤ÇÒÁ¹ÔÂÁÍÂÙèã¹Ëéͧ»¯ÔºÑµÔ¡ÒèØŪÕÇÇÔ·ÂҨӹǹÁÒ¡ã¹ »ÃÐà·Èä·Â 㹡Ò÷´Êͺ¤ÇÒÁ¶Ù¡µéͧáÅФÇÒÁáÁè¹ÂӢͧкºµÃǨÇÑ´ÃдѺ¤ÇÒÁäǵèÍÂÒµéÒ¹ ¨ØŪվ â´ÂÍÒÈÑÂÊÔè§Ê觵ÃǨ¨Ó¹Ç¹ 1526 µÑÇÍÂèÒ§ ¾ºÇèÒÃкº´Ñ§¡ÅèÒÇãËé¼Å¡ÒÃÇÔà¤ÃÒÐËì ÊÍ´¤Åéͧ¡Ñº¡ÒÃÇÔà¤ÃÒÐËì´éǼÙéàªÕèÂǪҭ´éǤèÒÊÑÁ»ÃÐÊÔ·¸ÔìÊËÊÑÁ¾Ñ¹¸ì 0.97 ÁÕ¤ÇÒÁ ¤ÅÒ´à¤Å×è͹¹éÍÂÁÒ¡ ÁÕ¤èÒ¤ÇÒÁäÇ»ÅÍÁ 0.5% áÅÐÁÕ¤èÒ¤ÇÒÁ´×éÍ»ÅÍÁ 0.2% «Öè§ÊèǹãË­èà»ç¹ ¼Å¨Ò¡¢éͼԴ¾ÅÒ´ÍѹÍÂÙè¹Í¡à˹×Í¡ÒäǺ¤ØÁÍÒ·Ôàªè¹ ¡ÒÃÁÕàª×éÍ»¹à»×é͹ ¡ÒÃÁÕá¹ÇÂѺÂÑ駷Ѻ «é͹ ËÃ×Íàª×éÍ·ÕèÁբͺࢵäÁèªÑ´à¨¹ ÏÅÏ

Abstract: The microbiological infection which is effectively controlled by antibiotic management is one of the most serious problems in Thailand. Many antibiotic susceptibility test were developed during this century and one of the most popular technique is Kirby Bauer’s Disc diffusion. The usefulness of Disk diffusion is its low operating cost, abilities to simultaneously test with many kind of antimicrobial agents, and potency for automatic test. The automatically process of susceptibility test not only produces the accurate and precise measurement by eliminating of measurement variation and human error but also brings on many advantage such as; large samples handling, technicians time reducing, laborious work reducing, and electronic data managing. The electrical data which compatible with Laboratory Information System are suitable for storing, archiving, and transferring through communication network in telemedicine objective. The development of automated susceptibility testing system consist of plate reader, video capture board and software which automatically measure and interpret the susceptibility results from inhibition zone around the antimicrobial disc. The measuring and interpreting processes are based on image processing principle such as image smoothening, edge enhancing, edge detection. This system also designed to work with database management system and work concomitantly with the popular MLAB system (well-known Thai antimicrobial susceptibility software) by importing patient data and sensitive criteria from MLAB and exporting the interpretation of susceptibility results back to MLAB later. The accuracy and precision of automated susceptibility testing system is tested by comparison with the expert manual interpretation of 1526 random specimens. The inhibition zone measured by developed system show high correlation with expert interpretation (r 0.97). The automatically interpretation demonstrates 0.5% false-susceptible and 0.2% false resistant which happen in case of uncontrollable situations such as contamination, superimposed area, ambiguous inhibition zone etc.

ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕ¾ÃШÍÁà¡ÅéÒ¾Ãй¤Ãà˹×Í. ÊӹѡËÍÊÁØ´¡ÅÒ§

Address: ¡Ãا෾ÁËÒ¹¤Ã

Email: library@kmutnb.ac.th

Name: ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕ¾ÃШÍÁà¡ÅéÒ¾Ãй¤Ãà˹×Í

Role: ¼ÙéãËé·Ø¹

Created: 2550

Modified: 2556-07-09

Issued: 2556-07-09

§Ò¹ÇÔ¨ÑÂ/Research report

application/pdf

CallNumber: E-RESEARCH

tha

©copyrights ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕ¾ÃШÍÁà¡ÅéÒ¾Ãй¤Ãà˹×Í

ÅӴѺ·Õè.	ª×èÍá¿éÁ¢éÍÁÙÅ	¢¹Ò´á¿éÁ¢éÍÁÙÅ	¨Ó¹Ç¹à¢éÒ¶Ö§	Çѹ-àÇÅÒà¢éÒ¶Ö§ÅèÒÊØ´
1	B15650960.pdf	4.45 MB	16	2021-02-08 12:07:16

ãªéàÇÅÒ

0.065506 ÇÔ¹Ò·Õ