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Pachuen Potup. Immunoglobulin class switching induced by plasmodium falciparum in vitro. Doctoral Degree(Tropical Medicine ). Mahidol University. Mahidol University Library and Knowledge Center. : Mahidol University, 2007.
Immunoglobulin class switching induced by plasmodium falciparum in vitro
การเกิด class switching ของ immunoglobulin โดยการกระตุ้นด้วยเชื้อ plasmodium falciparum ภายในห้องปฏิบัติการ
Name: Pachuen Potup
MeSH:
Immunoglobulins
MeSH:
Plasmodium falciparum
Abstract:
This in vitro study was designed by using normal human B cells stimulated
with crude Plasmodium falciparum (P. falciparum) antigens, to mimic the process of
immune cells being exposed to antigens released from lysed infected RBC in a natural
falciparum malaria infection. Human B cells from six healthy donors were divided in
2 groups for 2 studies. Each group was individually cocultured with 3 crude
P. falciparum antigens obtained from a Thai patient with uncomplicated malaria,
a cerebral malaria (CM) patient, and a laboratory isolate, and a normal RBC antigen
for 4 days, 11, 13 days, respectively. Positive control B cells from each donor were
cocultured with IL-4 and anti-CD40. Four days after incubation, RNA was extracted
from the cultured B cells and the occurrence of Ig class switch recombination (CSR)
from IgM to IgG and IgE was determined by detecting the presence of activationinduced
cytidine deaminase (AID) mRNA, germline gene transcripts (GLT), switch
circle transcripts (CT), γ mRNA and ε mRNA by conventional and real time reverse
transcription polymerase chain reaction (RT-PCR). Culture supernatants were
collected on day 11 and day 13 to measure for the content of IgM, IgG, and IgE by
ELISA assays.
The Ig gene expression results showed that CSR occurs in all stimulated
B cells but there were with different patterns of AID mRNA, GLT, CT, γ mRNA and
ε mRNA expression among the 3 donors and in the crude antigens. Gamma and
ε-GLTs, and γ mRNA expression were observed in all stimulated and unstimulated
B cells from all donors whereas the expression of ε mRNA, Iγ-C@ and Iε-C@ CT
varied among the different stimulation factors and donors. There was no occurrence
of CSR as indicated by AID mRNA expression and CT in unstimulated B cells. The
Ig production results showed that IgM and IgE production patterns varied between the
different stimulation factors, donors, and time period, while for there was no
significant differences in IgG production. In conclusion, the present study
demonstrated that crude P. falciparum antigens could stimulate CSR from IgM to IgG
and IgE and also induce IgM, IgG, and IgE production in human B cells in vitro.
Mahidol University. Mahidol University Library and Knowledge Center
Address:
NAKHONPATHOM
Email:
liwww@mahidol.ac.th
Role:
Thesis Advisors
Created:
2007
Modified:
2553-07-13
Issued:
2010-07-12
วิทยานิพนธ์/Thesis
application/pdf
CallNumber:
TH P116i 2007
eng
DegreeName: Doctor of Philosophy
Level: Doctoral Degree
Descipline: Tropical Medicine
Grantor: Mahidol University
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RightsAccess:
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0.228523 วินาที
Pachuen Potup
| Title | Contributor | Type |
|---|---|---|
| Immunoglobulin class switching induced by plasmodium falciparum in vitro
มหาวิทยาลัยมหิดล Pachuen Potup | วิทยานิพนธ์/Thesis | |
| Comparison of dose distribution for different techniques of breast irradiation treatment
มหาวิทยาลัยมหิดล Pachuen Potup | Sivalee Suriyapee Nopadol Asavametha Ratana Pirabul | วิทยานิพนธ์/Thesis |