Jinda Kungvanrattanasin. Growth curve inhibition of Entamoeba histolytica after exposure to Andrographis paniculata Nees. (whole plant), Euphorbia hirta L. (whole plant) and Lonicera japonica Thunb. (flowers and leaves) . Master's Degree(Infectious Diseases). Mahidol University. : Mahidol University, 2006.
Growth curve inhibition of Entamoeba histolytica after exposure to Andrographis paniculata Nees. (whole plant), Euphorbia hirta L. (whole plant) and Lonicera japonica Thunb. (flowers and leaves)
Abstract:
The purpose was to study the effect of herbal aqueous extract of A. paniculata
Nees.(whole plant), E. hirta L.(whole plant) and L. japonica thunb.(flowers and
leaves) to the growth of two strains of E. histolytica (AP and S) and one strain of E.
moshkovskii in vitro(xenic culture, biphasic media). The dried plants were extracted in
boiled water. Each aqueous extract of herbs was sterilized by being passed through
milipore and then mixed in aqueous media culture. Living amoebas were counted after
exposure to herb extract for 24 hours, and calculated for percentage inhibition.
Positive control was Metronidazole, 10 micrograms per milliliter.
The results revealed that undiluted herbal aqueous extract of L. japonica
Thunb. can kill three strains of Entamoeba trophozoites, but undiluted herbal aqueous
extract of E. hirta L. and aqueous extract of A. paniculata Nees. inhibits growth of
three strains of Entamoeba trophozoites. Undiluted herbal aqueous extract of E. hirta
L. had a growth inhibition effect on all three strains of Entamoeba trophozoites and
was more effective than undiluted herbal aqueous extract of L. japonica Thunb.. Three
kinds of herbal aqueous extracts had a growth inhibition effect on all three strains of
Entamoeba trophozoites; wheseas, growth inhibition effect decreased as the aqueous
extract was diluted.
The herbal aqueous extract of L. japonica Thunb.(flowers and leaves) can kill
Entamoeba trophozoite; wheseas, aqueous extracts of E. hirta L. (whole plant) and
A. paniculata Nees.(whole plant) can inhibit growth of Entamoeba trophozoites.