Phylogenetic analysis of polycyclic aromatic hydrocarbon degrading bacteria based on the nucleotide sequences of 16S rRna and gyrB genes

Name: Pumaree Kanrai

ThaSH: Phylogenetic tree

ThaSH: Maximum parsimony

Abstract: Phylogenetic tree showing the evoluationary relatedness of PAH-degrading bacteria including Stenotrophomonas maltophilia VUN 10010 ,Pseudomonas fluorescens VUN 10011 and Burkholderia sp. VUN 10013 based upon gyrB and 16S rRNA gene were compare . The result establishing the patterns of gyrB and 16SrRNA gene trees were congruent with the distribution of Pseudomonas fluorescens VUN 10011 on the tree revealed that this strain was closely related to other Pseudomonas ,especcially P.synxantha IFO03913 and P.marginalis NCPPB949. The position of Stenotrophomonas maltophilia VUN 10010 on the tree revealed that this strain was closely related to Xanthomonas special ,while Burholderia sp. VUN 10013 was phylogenetically close to other Burkholderria , especially B.caryophylli ATCC 25418. This result was in agreement with neighborjoining ,maxximum-parsimony,maximum-likelihood and Bayesian analysis, which method was suppost by high bootstrap. The phylogeneship determined by gyrB gene and GyrB protein sequence analyses were more consistent than those established by the 16SrRna phylogenetic three for Pseudomonas and Burkholderia spacies. Becuse of its high variability ,the phylogenetic analysis based on gyrB gene was more reliable than 16SrRNA to infer intragenetic relationship of Pseudomonas and Burkholderia species . The number of ncleotide substitution was significantlyhigher in the gyrB sequences than in the 6SrRNA sequences ,as had already been shown previously with member of taxa from different phyla. Comparison of nucleotide sequences with derived amino acid sequences indicated that the gyrB nucleotide sequences occured at the third position and did not affect the selection of amino acid. At present,16SrDNA sequences analysis was an acurated and rapid method for identifying most unknow bacteria to the genus level becuase the highly conserved gyrB sequences analysis was useful alternative to 16S rRNA analysis for construcing the phylogenetic relationship of bacteria ,in particular for the classified of closely related bacterial speies.

Burapha University. Library.

Address: ชลบุรี (Chonburi)

Email: buulibrary@buu.ac.th

Name: Sudarat Suanjit

Role: Principle advisor

Name: Wisatre Kongcharoensuntorn

Role: กรรมการ

Name: Voravit Cheevaporn

Role: กรรมการ

Created: 2548

Issued: 2548-06-28

Modified: 2549-11-30

วิทยานิพนธ์/Thesis

ISBN: 9745025291

tha

DegreeName: Master of Science

Level: Master's Degree

Descipline: Environmental Science

Grantor: Burapha University

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ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	title.pdf	367.79 KB	18	2018-05-19 23:14:36
2	chapter1.pdf	161.37 KB	9	2018-05-19 23:14:37
3	chapter2.pdf	2.38 MB	15	2018-05-19 23:14:38
4	chapter3.pdf	388.66 KB	8	2018-05-19 23:14:39
5	chapter4.pdf	1.45 MB	7	2018-05-19 23:14:39
6	chapter5.pdf	335.56 KB	5	2018-05-19 23:14:40
7	bibliogrphy.pdf	764.51 KB	5	2018-05-19 23:14:42
8	appendix.pdf	467.85 KB	5	2018-05-19 23:14:42

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