Abstract:
The research was aimed to study the comparison of malachite green biodegradation under aerobic, aerobic dentirifying, denitrifying and methanogenic conditions. Additionally, the sequencing steps for biodegradation of malachite green was established and minitored the results using HPLC, spectrophotometer and COD values. Comparison of 1 mM malachite green biodegradation using mixed culture of activated sludge under aerobic, aerobic denitrification, denitrification and methanogenic conditions was established. The color removal by aerobic, areobic denitrifying, denitrifying and methanogenic activated sludge was 19.05%, 55.07%, 35.42% and 43.33% , respectively. Therefore, aerobic denitrifying activated sluge was shown to be the best perfromance to degrade malachite green. In the next step, the sequencing technique for malachite green removal was conducted. Aerobic denitrifying activated sludge was set up to degrade malachite green within 7 days. Then the mathanogenic conditions for 7 days. The color of malachite green and its metabolites were detected through HPLC, decreasing af absorbance at 616.9 mm. And the reduction of COD. Obtained results showed that aerobic activated sludge represented the best technique for reducing the green color for 61.74% and the COD level 65.09%.