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Nisa Budda. Chitin Degradation by the Resting Cells of Chitinolytic Microorganism. (). Kasetsart University. Office of the University Library. : , ม.ค.-มิ.ย. 2547.
Chitin Degradation by the Resting Cells of Chitinolytic Microorganism
Name: Somchai Krairak
Organization :
King Mongkuts Institute of Technology Ladkrabang. Faculty of Science. Department of Applied Biology
Name: Nisa Budda
Organization :
Burapha University. Faculty of Science. Department of Microbiology
keyword:
chitinolytic microorganism
Abstract:
The chitinolytic microorganisms, isolates 18 and 19, were selected from soil samples around a region of frozen shrimp factory in Chonburi province. These isolates had high potential for using chitin as a carbon source from the chitin broth, which was composed of 1.0% chitin, 1.0% NH4NO3 and 3.0% yeast extract. The cultures were grown at 200 rpm, 30?C and initial pH of 6.8-7.0 for 7 days. It was found that the intact cells of isolates 18 and 19 had the chitinolytic activity of 19.51% and 20.31%, respectively. This study, the microorganisms were induced to transform the intact cells into the resting cells by freezethaw and ultrasonic method. The results showed that the resting cells which differentiated from the isolate 18 could be prepared by either 7 times of freeze-thaw or by 50% and 80% of sonicated amplitude for 35s and 15s, respectively. Additionally, the resting cells from these three methods had the chitinolytic activity about 31.01%, 25.06% and 25.01%, respectively. The preparation of the resting cells of isolate 19 was achieved by means of either 8 times of freeze-thaw or by 50% and 80% of sonicated amplitude for 70s and 35s, and yielded 24.78, 25.31 and 24.61% of chitinolytic activity, respectively
Kasetsart University. Office of the University Library
Address:
BANGKOK
Email:
tdckulib@ku.ac.th
Created:
ม.ค.-มิ.ย. 2547
Modified:
2552-10-10
Issued:
2009-10-10
บทความ/Article
application/pdf
วิทยาสารเกษตรศาสตร์ (สาขาวิทยาศาสตร์), 38(5) หน้า 36-44
eng
©copyrights Kasetsart University
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0.03018 วินาที
Somchai Krairak
| Title | Contributor | Type |
|---|---|---|
| Chitin Degradation by the Resting Cells of Chitinolytic Microorganism
มหาวิทยาลัยเกษตรศาสตร์ Somchai Krairak;Nisa Budda | บทความ/Article | |
| The Coenzyme Q10 production from Rhodopseudomonas sp. S12-13 mutant
มหาวิทยาลัยบูรพา Somchai Krairak;Nisa Krairak;สมชาย ไกรรักษ์;นิสา ไกรรักษ์ | บทความ/Article | |
| The pigment production from Monascus sp. U6V1, a Non-citrinin producing strain, in 5-L stirred tank fermentor
มหาวิทยาลัยบูรพา Somchai Krairak;Nisa Krairak;สมชาย ไกรรักษ์;นิสา ไกรรักษ์ | บทความ/Article |
Nisa Budda
| Title | Contributor | Type |
|---|---|---|
| Chitin Degradation by the Resting Cells of Chitinolytic Microorganism
มหาวิทยาลัยเกษตรศาสตร์ Somchai Krairak;Nisa Budda | บทความ/Article |