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Warangkana Chunglok. Cytotoxicity and Apoptotic Induction Properties of Tamarind Seed Extract in Human Oral Cancer Cell Line. (). WALAILAK UNIVERSITY. CENTER FOR LIBRARY RESOURCES AND EDUCATIONAL MEDIA. : , 2011-10-10.
Cytotoxicity and Apoptotic Induction Properties of Tamarind Seed Extract in Human Oral Cancer Cell Line
Name: Nantawan Utaipan
Email :
cwarang@wu.ac.th
Name: Mayura Somchit
Name: Yuttana Sudjaroen
Name: Warangkana Chunglok
keyword:
Tamarind seed extract
Abstract:
It has become evident that methanolic extract of Tamarind seed contains high contents of antioxidant
phytochemicals. The present study was conducted to investigate the anticancer activity of Tamarind seed extract in human
oral cancer cell line CLS-354. Cells were treated with crude methanolic extract of Tamarind Seed (TSE) for 24 h.
Cytotoxicity and apoptosis induction were investigated by MTT and AnnexinV-FITC/PI double staining assay, respectively.
The results showed that cell viability was significantly decreased upon TSE treatment at a concentration range from 160 -
400 μg/ml in comparison with control without TSE treatment (p < 0.05). TSE caused toxicity in CLS-354 cells with IC50
values of 400 μg/ml. Staining with AnnexinV-FITC and propidium iodide could induce apoptosis by 10 % at the dose of 75
μg/ml. Apoptotic cell death may due to alteration of apoptotic related proteins modulating by flavonoids in TSE. Thus,
anticancer activity of Tamarind seed extract may provide a new therapeutic agent for oral cancer treatment in the future.
WALAILAK UNIVERSITY. CENTER FOR LIBRARY RESOURCES AND EDUCATIONAL MEDIA
Address:
NAKON SI THAMMARAT
Email:
clm@wu.ac.th
Created:
2011-10-10
Modified:
2016-06-14
Issued:
2554-10-10
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